The RNA origin of transfer RNA aminoacylation and beyond
نویسندگان
چکیده
منابع مشابه
The RNA origin of transfer RNA aminoacylation and beyond.
Aminoacylation of tRNA is an essential event in the translation system. Although in the modern system protein enzymes play the sole role in tRNA aminoacylation, in the primitive translation system RNA molecules could have catalysed aminoacylation onto tRNA or tRNA-like molecules. Even though such RNA enzymes so far are not identified from known organisms, in vitro selection has generated such R...
متن کاملOrigin of amino acid homochirality: Relationship with the RNA world and origin of tRNA aminoacylation
The origin of homochirality of l-amino acids has long been a mystery. Aminoacylation of tRNA might have provided chiral selectivity, since it is the first process encountered by amino acids and RNA. An RNA minihelix (progenitor of the modern tRNA) was aminoacylated by an aminoacyl phosphate oligonucleotide that exhibited a clear preference for l- as opposed to d-amino acids. A mirror-image RNA ...
متن کاملStationary-phase expression and aminoacylation of a transfer-RNA-like small RNA.
Genome-scale analyses have shown numerous functional duplications in the canonical translational machinery. One of the most striking examples is the occurrence of unrelated class I and class II lysyl-transfer RNA synthetases (LysRS), which together may aminoacylate non-canonical tRNAs. We show that, in Bacillus cereus, the two LysRSs together aminoacylate a small RNA of unknown function named t...
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A chemically synthesized single-stranded ribonucleotide tridecamer derived from the 3' end of Escherichia coli alanine tRNA can be charged with alanine in the presence of short complementary RNA oligonucleotides that form duplexes with the 3' fragment. Complementary 5' oligomers of 9, 8, 6, and 4 nucleotides all confer charging of the 3' fragment. Furthermore, in the presence of limiting 5' oli...
متن کاملThe significance of 2' and 3' aminoacylation of RNA in the origin of amino acid homochirality.
It has been shown that the 2’ endoautoaminoacylation of surface-bound RNA is stereoselective for the Lenantiomers of the amino acids (1). This mimics selection of amino acids by RNA in an early ‘RNA world’ (2), and thus may have led to the evolution of L-amino acid homochirality. However, the current selection of amino acids for protein synthesis utilizes the 3’ exoaminoacylated species exclusi...
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ژورنال
عنوان ژورنال: Philosophical Transactions of the Royal Society B: Biological Sciences
سال: 2011
ISSN: 0962-8436,1471-2970
DOI: 10.1098/rstb.2011.0137